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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
16/12/2013 |
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Data da última atualização: |
10/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREIRA, G. S.; NUNES, E. S.; LAPERUTA, L. D. C.; BRAGA, M. F.; PENHA, H. A.; DINIZ, A. L.; MUNHOZ, C. F.; GAZAFFI, R.; GARCIA, A. A. F.; VIEIRA, M. L. C. |
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Afiliação: |
ESALQ/USP; ESALQ/USP; ESALQ/USP; MARCELO FIDELES BRAGA, CPAC; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP; ESALQ/USP. |
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Título: |
Molecular polymorphism and linkage analysis in sweet passion fruit, an outcrossing species. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Annals of Applied Biology, v. 162, n. 3, p. 347-361, 2013. |
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ISSN: |
0003-4746 |
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DOI: |
10.1111/aab.12028 |
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Idioma: |
Inglês |
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Conteúdo: |
One of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of which fit a segregation ratio of 1:1:1:1, 1.3% a ratio of 1:2:1, 27.3% a ratio of 3:1 and 69.4% a ratio of 1:1. The map spanned a total of 2073.0 cM, with an average distance between adjacent markers of 3.8 cM. This is the first linkage study on sweet passion fruit and should prove useful for quantitative trait loci mapping. MenosOne of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of w... Mostrar Tudo |
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Thesagro: |
Maracujá; Marcador genético; Marcador molecular; Melhoramento genético vegetal; Polimorfismo Genético. |
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Thesaurus Nal: |
Genetic improvement; Genetic markers; Genetic polymorphism; Passiflora alata; Passion fruits. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02903naa a2200373 a 4500
001 1973873
005 2014-12-10
008 2013 bl uuuu u00u1 u #d
022 $a0003-4746
024 7 $a10.1111/aab.12028$2DOI
100 1 $aPEREIRA, G. S.
245 $aMolecular polymorphism and linkage analysis in sweet passion fruit, an outcrossing species.
260 $c2013
520 $aOne of the current challenges of tropical fruit crop improvement is to incorporate molecular marker-based approaches into conventional breeding programmes. This study was designed to build an integrated genetic map of the sweet passion fruit (Passiflora alata), a diploid (2n = 18) outcrossing species which is greatly appreciated for in natura consumption, and reported to inspire cosmetic and pharmaceutical companies to create plant-derived compounds. With this in mind, a full-sib family of 180 individuals was genotyped using different molecular marker types, such as amplified fragment length polymorphisms (AFLP), microsatellite-AFLP (M-AFLP), simple sequence repeats (SSR), resistance gene analogues (RGA) and target region amplification polymorphism (TRAP). On average, the rate of polymorphism between the parental genotypes was 20.3%. We also searched for single nucleotide polymorphisms (SNPs) in some AFLP bands and in seven gene fragments, and found one SNP every 87 bp. All SNPs were biallelic and occurred most frequently in putative gene fragments (81.5%) rather than in AFLP bands (60.0%) analyzed. Excellent gel profiles were obtained allowing the recognition of all types of segregation expected for a progeny of an outcrossing species. Multipoint linkage analysis was performed using OneMap software, with logarithm of the odds (LOD) score ≥ 5.6 and recombination fraction <0.5. The resulting integrated map consists of 549 markers, 2.0% of which fit a segregation ratio of 1:1:1:1, 1.3% a ratio of 1:2:1, 27.3% a ratio of 3:1 and 69.4% a ratio of 1:1. The map spanned a total of 2073.0 cM, with an average distance between adjacent markers of 3.8 cM. This is the first linkage study on sweet passion fruit and should prove useful for quantitative trait loci mapping.
650 $aGenetic improvement
650 $aGenetic markers
650 $aGenetic polymorphism
650 $aPassiflora alata
650 $aPassion fruits
650 $aMaracujá
650 $aMarcador genético
650 $aMarcador molecular
650 $aMelhoramento genético vegetal
650 $aPolimorfismo Genético
700 1 $aNUNES, E. S.
700 1 $aLAPERUTA, L. D. C.
700 1 $aBRAGA, M. F.
700 1 $aPENHA, H. A.
700 1 $aDINIZ, A. L.
700 1 $aMUNHOZ, C. F.
700 1 $aGAZAFFI, R.
700 1 $aGARCIA, A. A. F.
700 1 $aVIEIRA, M. L. C.
773 $tAnnals of Applied Biology$gv. 162, n. 3, p. 347-361, 2013.
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Embrapa Cerrados (CPAC) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
25/11/2019 |
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Data da última atualização: |
25/11/2019 |
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Autoria: |
OLIVEIRA, L. M. de; MARCHESAN, E.; DAVID, R. de; WERLE, I. S.; ARAMBURU, B. B.; DONATO, G.; SILVA, A. L. da; COSTA, I. F. D. da. |
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Afiliação: |
Lillian Matias de Oliveira, Universidade Federal de Viçosa - UFV; Enio Marchesan, Universidade Federal de Santa Maria - UFSM; Ricardo de David, Universidade Federal de Santa Maria - UFSM; Isabel Schlegel Werle, Universidade Federal de Santa Maria - UFSM; Bruno Behenck Aramburu, Universidade Federal de Santa Maria - UFSM; Gabriel Donato, Universidade Federal de Santa Maria - UFSM; Anelise Lencina da Silva, Universidade Federal de Santa Maria - UFSM; Ivan Francisco Dressler da Costa, Universidade Federal de Santa Maria - UFSM. |
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Título: |
Occurrence of panicle blast in and grain quality of irrigated rice fertilized with nitrogen and silicates. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, v. 59, e00295, 2019 . |
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Idioma: |
Inglês |
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Notas: |
Título em português: Ocorrência de brusone e qualidade de grãos em arroz irrigado adubado com nitrogênio e silicatos. |
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Conteúdo: |
The objective of this work was to evaluate the effects of fertilization with nitrogen topdressing and silicates, as well as of the use of fungicides, on the occurrence of panicle blast, milling yield, and whole grains, vitreous grains, and chalky kernels in an irrigated rice cultivar sensitive to the disease. Two experiments were conducted in a randomized complete block design, in a 4×4×2 factorial arrangement, with four replicates: in experiment 1, four doses of nitrogen × four doses of calcium and magnesium silicate on soil × with and without fungicides; and, in experiment 2, four doses of nitrogen × four doses of potassium silicate applied on leaves x with and without fungicides. At the doses above 60 kg ha-1 nitrogen without fungicides, there was an increase in panicle blast severity, a decrease in the percentage of whole and vitreous grains, and an increase in chalky kernels and chalky area. The greatest effects of silicates occurred without fungicides. Fungicide use reduced the severity of blast in the panicles. Milling yield ranged from 60 to 70% in both experiments. The control of blast with fungicides maintains high rice grain quality, milling yield, and percentages of whole and vitreous grains. |
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Palavras-Chave: |
Grãos inteiros; Rendimento do grão; Whole grains. |
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Thesagro: |
Oryza Sativa; Pyricularia Oryzae. |
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Thesaurus NAL: |
Grain yield. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/205433/1/Occurrence-of-panicle-blast-in-and-grain.pdf
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Marc: |
LEADER 02184naa a2200289 a 4500
001 2115068
005 2019-11-25
008 2019 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, L. M. de
245 $aOccurrence of panicle blast in and grain quality of irrigated rice fertilized with nitrogen and silicates.$h[electronic resource]
260 $c2019
500 $aTítulo em português: Ocorrência de brusone e qualidade de grãos em arroz irrigado adubado com nitrogênio e silicatos.
520 $aThe objective of this work was to evaluate the effects of fertilization with nitrogen topdressing and silicates, as well as of the use of fungicides, on the occurrence of panicle blast, milling yield, and whole grains, vitreous grains, and chalky kernels in an irrigated rice cultivar sensitive to the disease. Two experiments were conducted in a randomized complete block design, in a 4×4×2 factorial arrangement, with four replicates: in experiment 1, four doses of nitrogen × four doses of calcium and magnesium silicate on soil × with and without fungicides; and, in experiment 2, four doses of nitrogen × four doses of potassium silicate applied on leaves x with and without fungicides. At the doses above 60 kg ha-1 nitrogen without fungicides, there was an increase in panicle blast severity, a decrease in the percentage of whole and vitreous grains, and an increase in chalky kernels and chalky area. The greatest effects of silicates occurred without fungicides. Fungicide use reduced the severity of blast in the panicles. Milling yield ranged from 60 to 70% in both experiments. The control of blast with fungicides maintains high rice grain quality, milling yield, and percentages of whole and vitreous grains.
650 $aGrain yield
650 $aOryza Sativa
650 $aPyricularia Oryzae
653 $aGrãos inteiros
653 $aRendimento do grão
653 $aWhole grains
700 1 $aMARCHESAN, E.
700 1 $aDAVID, R. de
700 1 $aWERLE, I. S.
700 1 $aARAMBURU, B. B.
700 1 $aDONATO, G.
700 1 $aSILVA, A. L. da
700 1 $aCOSTA, I. F. D. da
773 $tPesquisa Agropecuária Brasileira$gv. 59, e00295, 2019 .
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